Abstract
We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA by 454 pyrosequencing yielded approximately 250,000 expressed sequence tags (ESTs) from one run of sequencing. The data also showed marked heterogeneity of the 5′ ends of the transcripts, including the addition of non–template-encoded bases.
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