Abstract
A high-performance liquid chromatographic system was developed for the analysis of ten selected β-carbolines. It consists of tri- n-butyl phosphate as liquid stationary phase and buffered mixtures of water and methanol with perchlorate as the mobile phases. The separation was optimized by means of mobile phase parameters such as the perchlorate concentration, the methanol content and the pH. The relationship between these parameters and the retention of the β-carbolines was determined and compared with theory as derived from a model of the retention mechanism. The potential of spectrofluorometric and UV spectroscopic detection with regard to the detection limits, linear dynamic range and selectivity were investigated.
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