Abstract
A method for the analysis of lysine using a specific decarboxylase has been developed. The amine resulting from decarboxylation is reacted with dinitrofluorobenzene. The product is selectively extracted into chloroform and determined by spectrophotometry at 400 mμ. The sensitivity of the method is at least one hundred times greater than manometric assay using the same enzyme. Lysine in blood plasma and urine may be easily measured at physiological levels. The method has been extended to tyrosine, arginine, and histidine, and data are presented for its extension to the amine analogs of several other amino acids.
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