Analysis, Chemotype and Quality Control of the Essential Oil of a New Cultivated Basil (Ocimum basilicum L.) Plant from Bulgaria

Leopold Jirovetz,Anastasia Balinova,Albena Stoyanova,Gerhard Buchbauer

doi:10.3797/scipharm.aut-01-10

Leopold Jirovetz, Anastasia Balinova + Show 2 more

Open Access

https://doi.org/10.3797/scipharm.aut-01-10

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Abstract

The essential oil of the spice plant Ocimum basilicum L. (Lamiaceae) from Bulgaria was investigated using gas chromatographic-spectroscopic (GC/FID and GC/MS: polar, non-polar and chiral phases of GC columns) and olfactoric methods. As main compounds of this basil oil sample (R)-(-)-linalool (71.4%), (Z,E)-α-farnesene (6.5%), 1,8-cineole (5.6%), τ-cadinol (2.8%), germacrene D (1.7%), citronellol (1.2%) and bornyl acetate (1.1%) were identified. Ths results show, that the essential basil oil from Bulgaria belongs to the European linalool chemotype. The correlations of olfactoric data (qualitative and quantitative) with the presented analytical ones certificate the good quality of this Bulgarian Ocimum basilicum sample. A possible use of this essential basil oil in medicine, perfumery and foods is discussed.

Highlights

Solid lipid nanoparticles (SLN) are attractive colloidal drug carriers, which combine advantages of polymeric nanoparticles, liposomes and fat emulsions but simultaneously avoid some of their disadvantages
The interaction of SLN with leukocytes was determined from the EPR spectra intensity measurements with time of incubation of leukocytes with spin-labelled derivatives (SP) loaded SLN, from the reduction kinetics of the SP due to the oxy-redoxy systems in leukocytes, from reoxidation of the reduced SP by K I F ~ ( C N )a~nd by computer simulation of the EPR spectral line shapes, Intensity measurements show directly the amount of SP transferred from SLN to leukocytes, while from the reduction kinetics and by computer simulation it is possible to distinguish if there is an exchange of SP between SLN and leucocytes or endocythosis of SLN is involved
Our results show that the leukocytes viability in PBS during the three hours period does not change, while after addition of SLN dispersion (40 mg SLNI mL cell) it decreases for s 10%

Summary

INTRODUCTION

Solid lipid nanoparticles (SLN) are attractive colloidal drug carriers, which combine advantages of polymeric nanoparticles, liposomes and fat emulsions but simultaneously avoid some of their disadvantages. SLN were spin labelled by PC(10,3) or MeFASL(10,3) at the ratio of spin probe to lipids 1 : 10 At this concentration of spin probe the broadening of the EPR spectra lines is observed due to the spin-spin exchange. The interaction of SLN with leukocytes was determined from the EPR spectra intensity measurements with time of incubation of leukocytes with SP loaded SLN, from the reduction kinetics of the SP due to the oxy-redoxy systems in leukocytes, from reoxidation of the reduced SP by K I F ~ ( C N )a~nd by computer simulation of the EPR spectral line shapes, Intensity measurements show directly the amount of SP transferred from SLN to leukocytes, while from the reduction kinetics and by computer simulation it is possible to distinguish if there is an exchange of SP between SLN and leucocytes or endocythosis of SLN is involved

RESULTS AND DISCUSSION

CONCLUSIONS

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