Abstract

Fast atom bombardment (FAB) technique was employed to determine the structure of polar lipids from the cellular membrane of Lactobacillus delbruekii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus. Analysis of spectra provided useful information about the molecular species and aminoacids constituents of the samples.

Highlights

  • Lactobacillus delbruekii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus, thermophilic lactic acid bacteria, are used as starter cultures for the manufacture of yogurt and different types of cheeses on a worldwide scale

  • As lipids are important in maintaining cell membrane structure of Gram-positive microorganisms it would be of interest to know whether the different types of lipids and the fatty acids distribution on them are involved in the cell membrane integrity during freezing

  • The polar lipids of thermophilic acid lactic bacteria were characterized by their characteristic migrations on thin-layer chromatographic (TLC) plates

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Summary

Introduction

Lactobacillus delbruekii ssp. bulgaricus and Streptococcus salivarius ssp. thermophilus, thermophilic lactic acid bacteria, are used as starter cultures for the manufacture of yogurt and different types of cheeses on a worldwide scale. Thermophilus, thermophilic lactic acid bacteria, are used as starter cultures for the manufacture of yogurt and different types of cheeses on a worldwide scale. The high sensitivity of these microorganisms to cryogenic treatments results in structural and physiological injury that makes their preservation difficult. Both the bacterial cell wall and membrane are damaged after freezing-thawing processes [1].

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