Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS

Abstract

To investigate the monoterpene glycosides in Paeonia lactflora by UPLC-MS/MS. An Acquity UPLC BEH C18 column (2.1 mm x 50 mm) with 1.7 microm particle size was used. The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode. The flow rate was 0.4 mL x min and the chromatographic run time was 9 min for one run. The mass spectrometer equipped with an eletrospray ion source in negative ion mode. Totally six glycosides were analyzed and identified by the established UPLC-MS/MS method. The method was rapid, sensitive, and extremely useful for rapid identification of glycosides in P. lactiflora.