Abstract
6 strains of Pyropia yezoensis( Ueda) M. S. Hwang et H. G. Choi were analyzed using SRAP (sequence-related amplified polymorphism) markers in order to identify the germplasm. DNAs from conchocelis of 6 strains were screened with 35 primer combinations,of which 11 primer combinations gave stable and reproducible amplification patterns. Among the total 131 fragments,125 fragments( 95.42%) were polymorphic. The genetic distances of 6 strains were between 0.3643 and 0.8679,and the average was 0.5930. Cluster analysis of UPMGA showed a good and true relationships among the 6 strains. The results demonstrated that SRAP could be a useful tool in germplasm identification of P. yezoensis strains. From the total 131 fragments,4 fragments amplified by 1 primer combination were used to develop the DNA fingerprints of 6 strains of P. yezoensis. One specific fragment of TM-18 was obtained in the fragments amplified by primer combination ME1/EM6. According to the DNA sequence of the fragments,a specific pair-primer was designed and it achieved stable amplification of a 340bp specific band in both DNA of conchocelis and blades of TM-18. The specific marker could be used in fast identification of TM-18 strain.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
