Abstract
During the last decade, our understanding about the function and biological activity of metabolites has drastically changed. Metabolites previously seen as sole bystanders of biochemical reactions without unique activity are becoming more and more recognized for their biological functions. One class of metabolites for which such a paradigm shift has become evident is cholesterol and its precursors. Here, we will give a brief overview about the post-squalene pathway of cholesterol biosynthesis, its inhibition, the biological functions of its intermediates and put special emphasis on the analysis of the occurring metabolites. We will compare liquid chromatography and gas chromatography-based analysis platforms, describe mass spectrometric fragmentations and explain different derivatization strategies. In addition, we will present a full spectral data set of 27 analytes of which 24 are sterols, synthesized, isolated and characterized in several studies in our laboratories. Finally, with our overview, we hope to assist researchers in the field of cholesterol biosynthesis with the chemical analysis of cholesterol and its physiological as well as non-physiological precursors.
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