Abstract
A reversed phase high performance liquid chromatographic method combined with atmospheric pressure chemical ionization mass detection (LC/APCI-MS) has been developed for the separation and analysis of sophorolipids produced byC. bombicola when grown on fatty acid mixtures. Using this method it was found that the incorporation of palmitic, linoleic, and linolenic acids into the sophorolipid structure was dependent on the initial fatty acid, content of these acids, whereas the incorporation of oleic acid, was independent of its initial content in the mixture. Also observed was the incorporation of esterified glycerol into the sophorolipid structure, which has not been reported previously.
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