Abstract

AbstractThe chemical composition and antitumor activity of essential oil were compared for four parts of the Arisaema Amuremse Maxim. Chemical constituent of essential oil in every parts of Arisaema Amuremse Maxim was identified by GC-MS analysis systematically. Arisaema Amuremse Maxim had 114 kinds of essential oils. Tubers, petioles, leaves, and fruits were identified 53, 48, 5 and 21 species essential oil, respectively. The antiproliferative activity against Hep2, HCT-116, A549, SW480, HepG2 cell lines had been investigated by MTT assay. The essential oil of tubers, petioles, leaves and fruits all had strong antiproliferative activity. The IC 50 was 19.60μg·mL-1, 17.60μg·mL-1, 23.80μg·mL-1, and 30.23μg·mL-1, respectively. Among them, the essential oil of tubers and petioles had stronger antitumor activity. The essential oil of tubers, petioles, leaves and fruits had strong antiproliferative activity. This study was of great significance to increase the medicinal parts of Arisaema Amuremse Maxim.

Highlights

  • IntroductionPetioles, leaves, and fruits were identified 53, 48, 5 and 21 species essential oil, respectively

  • The chemical composition and antitumor activity of essential oil were compared for four parts of the Arisaema Amuremse Maxim

  • 100 g dry tubers, petioles, leaves, fruits were put in the different essential oil extractor, 1000 ml distilled water were added to extract for 10 hours

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Summary

Introduction

Petioles, leaves, and fruits were identified 53, 48, 5 and 21 species essential oil, respectively. The essential oil of tubers, petioles, leaves and fruits had strong antiproliferative activity. Arisaema Amuremse Maxim, called aliased corn and snake corn, is traditional Chinese perennial herb medicine, mainly distributed in Heilongjiang, Jilin and Liaoning Provinces of China, as well as in Korea and Russia [1]. It is bitter in taste, pungent, warm, poisonous, and effects on fight swelling [2,3]. We used steam distillation to extract the tubers, petioles, leaves, and fruits of Arisaema Amuremse Maxim and obtained the essential oil. The Arisaema Amuremse Maxim was adopted and identified by Professor Chendi of the School of Pharmacy of Jilin University in the Changbai Mountain area of Jilin Province in 2016

Extraction and Identification
GC-MS chromatographic conditions
Cell culture
Results and discussion
The antiproliferative activity was investigated by MTT assay
Discussion
Conclusions

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