Analysing the DNA damage and replication checkpoints in DT40 cells

Abstract

Eukaryotic cells respond to DNA damage or blocks to DNA replication by triggering a variety of "checkpoint" responses which delay cell cycle progression, modulate DNA replication, and facilitate DNA repair. Checkpoints play a vital role in maintaining genome integrity, particularly under conditions of genotoxic stress, and mutations in checkpoint genes can predispose to cancer and aging. Checkpoints are best understood at the molecular level in model organisms such as fission yeast, where the presence of aberrant DNA structures is sensed and relayed via signal transduction pathways to activate the checkpoint effector kinases, Chk1 and Cds1/ Chk2, which implement appropriate responses. Many of the yeast checkpoint sensor, transducer, and effector proteins are conserved in vertebrate cells, raising the question of whether they function in a similar or analogous way. DT40 cells provide a particularly tractable experimental system for genetic and biochemical dissection of checkpoints in vertebrates. Thus far, gene knockouts in DT40 have revealed that the Chk1 and Chk2 checkpoint effector kinases control a very different range of checkpoint responses in vertebrates compared to yeast. In future, these and other DT40 mutants will provide powerful tools for understanding the molecular basis of these unexpected differences and detailed studies of checkpoint mechanisms.