Abstract
PCR-based genic and microarray-based Diversity Arrays Technology (DArTTM) markers were used to determine genetic diversity in 94 accessions of white lupin (Lupinus albus L.) comprising Australian and foreign cultivars, landraces, and advanced breeding lines from Australian breeding programs. A total of 345 (50 PCR-based and 295 DArT-based) polymorphic fragments were identified, which were used to determine the genetic diversity among accessions. Both cluster analysis of bivariate marker data using UPGMA, and principal coordinate analysis, indicated a high level of genetic diversity in the germplasm. Our results showed that both types of markers used in this study are suitable for estimation of genetic diversity. Landrace accessions from Ethiopia formed a very distinct and separate grouping with both marker systems. Australian cultivars and breeding lines were clustered together and tended to be distinct from European landraces. These findings will allow breeders to select appropriate, diverse parents to broaden the genetic base of white lupin breeding populations.
Highlights
Lupinus albus L., a member of the Leguminosae, is an annual grain-legumeHow to cite this paper: Raman, R., et al (2014) Analyses Using SSR and DArT Molecular Markers Reveal that Ethiopian Accessions of White Lupin (Lupinus albus L.) Represent a Unique Genepool
The availability of a suite of markers based upon structural and functional genes [22] plus a DArT lupin chip, provided an opportunity to assess the genetic diversity and population structure in the germplasm available to the white lupin breeding program at Wagga Wagga, and the opportunity to compare the results from the two marker systems
The bulk sampling procedure [20] was followed as an efficient way to determine genetic diversity both between and within germplasm accessions
Summary
DArTTM markers are microarray based and are amenable to high-throughput genotyping and are cost-effective per data point, making them suitable for screening large number of individuals These markers have been employed for genetic fingerprinting and diversity assessment, molecular mapping of different genomes, and development of marker-trait associations in several crops including: wheat, barley, cassava, canola, rice, and white lupin [24] [26]-[31]. The availability of a suite of markers based upon structural and functional genes [22] plus a DArT lupin chip, provided an opportunity to assess the genetic diversity and population structure in the germplasm available to the white lupin breeding program at Wagga Wagga, and the opportunity to compare the results from the two marker systems
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