Analyses ofElaeocarpus sphaericusextract for antioxidant, anti-proliferative and gene repression activities against HIF-1 alpha and VEGF.

Abstract

The study presents antioxidant, phytochemical, anti-proliferative, and gene repression activities against Hypoxia inducible factor (HIF-1) alpha and Vascular endothelial growth factor (VEGF) ofElaeocarpus sphaericusextract. Total phenolic content (TPC) and total flavonoid content (TFC) were used to measure the extracts' phytochemical activity (TFC). Antioxidant potential of the extracts was measured through DPPH, ABTS, FRAP, and TRP. Methanol extract of the leaves ofE. sphaericushas shown higher amount of the TPC (94.666±4.040 mg/gm GAE) and TFC value (172.33±3.21 mg/gm RE). The antioxidant properties of extracts in the yeast model (Drug Rescue assay) showed promising results. Ascorbic acid, gallic acid, hesperidin, and quercetin were found in the aqueous and methanolic extracts ofE. sphaericusat varying amounts, according to a densiometric chromatogram generated by HPTLC analysis. Methanolextract ofE. sphaericus(10 mg/ml) has shown good antimicrobial potential against all bacterial strains used in the study exceptE. coli.The anticancer activity of the extract in HeLa cell lines ranged from 77.94±1.03% to 66.85±1.95% while it ranged from 52.83±2.57% to 5.44% in Vero cell lines at varying concentration (1000µg/ml-31.2 µg/ml). A promising effect of extract was observed on the expression activity of HIF-1 and VEGF gene through RT-PCR assay.