Abstract
In order to investigate the effect of pollination type on seed formation of Gazania rigens L.,gazania was chose as experimental materials, pollen coat and its components were analyzed. The results showed that, (1) there existed β-carotene on yellow pollen surface of gazania was, for it had the same Rf value as standard sample of β-carotene analyzed by plate chromatography. (2) analyzed by pollen surface protein colour spectrum -mass spectrometry, pollen surface proteins were mainly 30-50 kD.(3) by searching the NCBI protein database, 22 kinds of protein were compared and three of them were reliable. (4)these peptides with kinesin motor domain ,participate motion and spindle elongation of chromosome during mitosis. These results provide theoretical basis for affinity mechanism research of self-fertilization and cros-fertilization of Compositae plants.
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