Abstract
The aim of this study was to evaluate the surface free energy (SFE), wetting and surface properties as well as antimicrobial, adhesion and biocompatibility properties of diamond-like carbon (DLC)-coated surfaces. In addition, the leakage of Escherichia coli through the abutment-dental implant interface was also calculated. SFE was calculated from contact angle values; R a was measured before and after DLC coating. Antimicrobial and adhesion properties against E. coli and cytotoxicity of DLC with human keratinocytes (HaCaT) were evaluated. Further, the ability of DLC-coated surfaces to prevent the migration of E. coli into the external hexagonal implant interface was also evaluated. A sterile technique was used for the semi-quantitative polymerase chain reaction (semi-quantitative PCR). The surfaces showed slight decreases in cell viability (p<0.05), while the SFE, R a, bacterial adhesion, antimicrobial, and bacterial infiltration tests showed no statistically significant differences (p>0.05). It was concluded that DLC was shown to be a biocompatible material with mild cytotoxicity that did not show changes in R a, SFE, bacterial adhesion or antimicrobial properties and did not inhibit the infiltration of E. coli into the abutment-dental implant interface.
Highlights
Diamond-like carbon (DLC) is the basis of many studies due to its mechanical, physical and chemical properties, as well as its wide range of applications in electronics and in chemical, mechanical- and bio-engineering
Surfaces coated with diamond-like carbon (DLC) were evaluated because the technique has recently attracted attention due to its unique physical, chemical and mechanical properties and it may be applied in many different areas of health care, including dentistry
The samples were coated with DLC by CVDentus® by means of technical chemical vapor deposition (CVD)
Summary
Diamond-like carbon (DLC) is the basis of many studies due to its mechanical, physical and chemical properties, as well as its wide range of applications in electronics and in chemical-, mechanical- and bio-engineering. The use of implant-supported prostheses for the treatment of partially or totally edentulous patients has shown success, complications with singleunit prostheses may result in loosened screws, which can cause mechanical complications and inflammation in periimplant tissue and eventually the loss of the implant. If exacerbated, it promotes the infiltration of bacterial flow, regardless the size of the interface between abutments and dental implants and the type of prosthetic connection [10]. Previous reports have shown that protein adsorption and bacterial adhesion in vivo may result by a threshold surface roughness of 0.2 μm [12]
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