Analyses chimiques des polyosides extraits de Salmonella johannesburg 5.58 sauvage et convertie par le phage ϕ 1(40)

Abstract

After conversion by phage ϕ 1(40) S. johannesburg 5.58 (40) acquires a new specificity which cross reacts with all Factors 1 present on other Salmonella. The structure of the specific polysaccharides, obtained from both wild (polysaccharide R −) and converted (polysaccharide R +) strains, was established by determination of the component sugars and by methylation and periodate oxidation of the polysaccharides and a few oligosaccharides obtained after partial hydrolysis. The specific chain of polysaccharide R − contains residues of D-glucose and D-mannose, and a tetrasubstituted residue of 2-acetamido-2-deoxy- D-galactose on which two residues of 2-acetamido-2-deoxy- D-glucose and - D-galactose are linked. The specific chain of polysaccharide R + apparently differs only from that of polysaccharide R - by the addition of an α- D-glucose residue to the 2-acetamido-2-deoxy- D-galactose residue. The terminal disaccharide residue α- D-Glc-(1→6)- D-GalNAc of polysaccharide R + is similar to the residue α- D-Glc-(1→6)- D-Gal which carries the specificity of Factor 1 in other Salmonella. Both the very small quantities of 2,3,4,6-tetra- O-methyl- D-glucose obtained after methylation and the resistance of the terminal residue of 2-acetamido-2-deoxy- D-glucose to periodate oxidation cannot be explained by the structure proposed for polysaccharide R +, unless by a special conformation which is discussed and is presently tested by immunochemical analysis.