Abstract
Objectives: Blood cultures are the primary and the most sensitive method for diagnosing blood stream infections (bacteriemia, fungemia, and sepsis) and sensitivity to antibiotics. However, contamination may impact on patients care and lead to increased patient stay, additional tests, and inappropriate antibiotic use. Aim: The objective of this study was to calculate the rate of contamination and assess possible associated factors Methods: This study is a quantitative observational retrospective cross selection study. The total study population was calculated based on a review of all of the request sheets for blood cultures submitted to the microbiology laboratory from 1st of September to 31st of December, 2016, at The Institute for health protection of children and youth Vojvodine. Results: The rate of blood culture contamination (falsepositive) amounted to 4.93%. The most common isolated pathogen was Staphylococcus spp. in 29 cases, then Klebseilla pneumonie, Streptococcus viridans and beta-hemolytic streptococcus G. Analysis of the data revealed that the departments with the highest number of cases of contamination of blood culture department of neonatology and intensive care. Conclusion: The rate of blood culture contamination is a required question the quality of most hospitals. The overall rate of blood culture contamination at the Institute for Health Protection of Children and Youth of Vojvodina in Novi Sad, is not within the acceptable international range.
Highlights
Rezultati studije sprovedene 2012. godine u istoj ustanovi ukazuju na smanjenje stope kontaminacije nakon uvođenja ček liste i sterilne tehnike uzorkovanja sa 16.4% na 7.6% (6)
Na osnovu analiziranih podataka stopa kontaminacije hemokultura na Institutu za zdravstvenu zaštitu dece i omladine Vojvodine 4.93%, što je više od međunarodnog prihvaćenog opsega
Summary
Uzorkovanje krvi za hemokulturu je dijagnostički postupak koji se često primenjuje u kliničkoj praksi i može da pruži osnovne informacije o različitim zaraznim bolestima, uključujući septikemiju ili sepsu (1). Primarni preanalitički faktori koji dosta utiču na senzitivnost, interpretaciju i kliničku relevantnost hemokulture su antiseptička priprema kože, volumen uzorka krvi, vreme uzorkovanja, transport i zadržavanje u inkubacionom periodu (3). Lažno pozitivni rezultati hemokultura se često javljaju kao posledica kontaminacije uzoraka (1). Pre samog uzorkovanja krvi za hemokulturu, neophodno je sačekati da se mesto gde je nanet antiseptik osuši (4).
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