Analisis Kariotipe Ular Trawang (Coelognathus radiatus, (Boie 1827))

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiated ratsnake is a reptile which has an economic potential an export comodyty. However, there are few reports on genetic studies of radiated ratsnake. The aim of this research was to examine chromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah Istimewa Yogyakarta. Chromosome preparation method used was splash using blood culture. The results revealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified as metacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric (chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15). Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. The result showed that the shortest of short arm chromosomes was 0 ?m, the longest one was 0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longest one was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, and the longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408 indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta has chromosome variation size. Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype