Abstract
This research aimed to analyze the levels of vitamin C and β -carotene in Moringa leaves extract (Moringa oleifera Lam. ) using UV-Vis spectrophotometry. Vitamin C on Moringa leaves are extracted with ethanol 96%. The extracts obtained was qualitatively analyzed of levels of vitamin C using specific reagents an iodine 1% and ammonium molybdate 5%. Than quantitatively using UV-Vis spectrophotometry at wavelength of 570 nm, obtained an verage grade of 7,96 mg/g. For β -carotene, Moringa leaves were extracted with acetone. The extract obtained was extracted again with ether, then saponified with KHO solvent 15% w/v in methanol, allowed to stand overnight. The extract obtained was extracted again with ether, then freed from wet with distilled water. Extracts were analyzed qualitatively using thin layer chromatography and quantitatively using UV-Vis spectrophotometry at wavelength of 450 nm and obtained an average grade of 3,31 mg/g.
Highlights
Indonesia memiliki potensi sumber daya alam yang sangat berlimpah baik yang berasal dari hewan maupun dari tanaman yang dapat dijadikan sebagai sumber makanan ataupun obat-obatan
This research aimed to analyze the levels of vitamin C and β-carotene in Moringa leaves extract
Vitamin C on Moringa leaves are extracted with ethanol 96%
Summary
Indonesia memiliki potensi sumber daya alam yang sangat berlimpah baik yang berasal dari hewan maupun dari tanaman yang dapat dijadikan sebagai sumber makanan ataupun obat-obatan. 3. Pembuatan kurva baku Larutan asam askorbat 1000 ppm dipipet sebanyak 7 kali yaitu 0,2; 0,3; 0,4; 0,5; 0,6; 0,7 dan 0,8 mL, masing-masing dimasukkan kedalam labu ukur 10 mL, kemudian ditambahkan H2SO4 5% sebanyak 4 mL lalu dicukupkan volumenya dengan ammonium molibdat 5% sampai batas tanda, dikocok dan dihomogenkan (diperoleh konsentrasi 20; 30; 40; 50; 60; 70 dan 80 ppm), kemudian diinkubasi selama 30 menit.
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