Abstract

Analysis of active compounds in fermented plant extract (FPE) from the ngapi nut peel using Effective Microorganism-4 (EM-4) has been done by Gas Chromatography-Mass Spectroscopy (GC-MS). There were 88 compounds found in fermented plant extract, the major content with the area above 1% were 7 compounds such as succinic acid; ethyl hydrogen succinate; 1,2,3-benzetriol; 3,3-dimethylhexanal; L-proline, 1-methyl-5-oxo-methyl ester; 3-methoxycinnamic acid and hexadecanoic acid. Based GC-MS analysis, fermented plant extract indicated that several compounds that have potential as biopesticides.

Highlights

  • from ngapi nut peel can be used as a biopesticide on tomato plants

  • tomatoes plant treated with Fermented Plant Extract (FPE) could increase resistance

  • active compounds that play a role as biopesticides in FPE

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Summary

METODOLOGI PENELITIAN Bahan dan alat

Bahan utama yang digunakan dalam penelitian ini adalah kulit buah jengkol. Bubuk kulit jengkol disimpan dalam wadah kering dan tertutup untuk digunakan dalam pekerjaan selanjutnya. Pembuatan Ekstrak Tanamana Terfermentasi (ETT) dari Kulit Buah Jengkol Sebanyak 50 g bubuk kulit buah jengkol ditimbang dan kemudian difermentasi dengan 25 mL EM-4, 25 mL gula aren (1kg/L) dan 450 mL air suling dalam botol plastik selama ± 15 hari, tutup botol dibuka setiap hari untuk mengeluarkan gas yang terbentuk. Analisis GC-MS Ekstrak Tanaman Terfentasi dari Kulit Buah Jengkol Kandungan senyawa metabolit sekunder dalam ekstrak tanaman terfermentasi dari kulit buah jengkol diidentifikasi dengan menggunakan Agilent GC-MS 19091S-433, kolom HP-5MS 30m x 250 μm x 0,25 μm, suhu oven (0°C325°C), Antarmuka (250°C), Mode kontrol (split), tekanan (10.523 psi), total aliran (104 mL/menit) ), split ratio (100:1), split flow (100 mL/mnt), gas (He) dan detektor (MSD)

HASIL DAN PEMBAHASAN Hasil Uji Fitokomia ETT Kulit Buah Jengkol
Asam heksadekanoat
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