Abstract

Angelicae Tenuissimae Radix (ATR) has traditionally been used for flu-like symptoms, limb-ache and disability, and even for toothache. In the present study, anti-inflammatory and analgesic effect of ATR was investigated on lipopolysaccharide-induced inflammation was investigated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot, prostaglandin E2 (PGE2) immunoassay, and nitric oxide (NO) detection in mouse BV2 microglial cells. In addition, the effect of ATR on carrageenan-induced edema, acetic acid-induced abdominal pain, and heat-induced hyperalgesia were investigated using rats and mice. The present results showed that ATR suppressed PGE2 synthesis and NO production by inhibiting LPS-induced expressions of cyclooxygenase-2 and inducible nitric oxide synthase mRNA and protein in mouse BV2 microglial cells. ATR reduced carrageenan-induced edema in rats and inhibited acetic acid-induced abdominal pain in mice. Here in this study, we have shown that ATR possesses anti-inflammatory and analgesic effects. This study was supported by a grant of the Oriental Medicine R&D Project, Ministry of Health & Welfare, Republic of Korea (0405-OD00-0815-B050049).

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