Abstract
Objective To evaluate the analgesic effects of adenovirus-mediated IL-24 (Ad-IL-24) gene transfection in rats with tibial cancer pain. Methods QBI-293A cells were transfected with Ad-IL-24 and Ad-GFP respectively according to Wang et al. High titer adenovirus was acquired after being amplified many times. Thirty-two female SD rats weighing 180-200 g were randomly divided into 4 groups (n = 8 each) :group Ⅰ normal control; group Ⅱ PBS; group Ⅲ Ad-GFP and group Ⅳ Ad-IL-24. Bone cancer was induced by injecting Walker 256 cancer cells into left tibia. On the 9th day after cancer cell inoculation, 70 μl of PBS, Ad-GFP and Ad-IL-24 were injected subcutaneously into the operated hindleg once every other day×3 times respectively. Pain threshold to noxious mechanical stimulation was measured before (baseline) and on days 3, 6, 8, 10, 12, 14 after cancer cell inoculation. The operated legs were then cut and stained with haematoxylin and eosin (HE) for examination of tumor infiltration and bone destruction. Plasma concentrations of TNF-α, IL-6, β-endorphin and IFN-γ were measured on the 14th day after cancer cell inoculation. Results Mechanical pain threshold was significantly decreased after cancer cell inoculation in group Ⅱ-Ⅳ as compared with the baselines and control group. Administration of Ad-IL-24 significantly decreased mechanical hyperalgesia induced by cancer cell inoculation in group Ⅳ as compared with PBS and Ad-GFP groups (group Ⅱ and Ⅲ). Plasma concentrations of IFN-γ, β-endorphin and TNF-α were significantly higher, while plasma IL-6 concentration was significantly lower in group Ad-IL-24 than in group PBS and Ad-GFP. In group PBS and Ad-GFP the tumor destroyed bone matrix and periosteum and grew out of the bone. Repeated Ad-IL-24 administration ameliorated the damage caused by cancer. Conclusion Ad-IL-24 gene transfection can decrease the degree of tibial cancer pain induced by Walker 256 cell inoculation through suppression of the growth of tumor and modification of cytokine secretion. Key words: Bone neoplasms; Pain; Transfection; Analgesia; Adenovifidae; Interleukins
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
