Analgesic activity of Terminalia bellerica fruit pulp aqueous extract by using acetic acid induced writhing test in Swiss albino mice by peripheral mechanism

Abstract

Objective: To evaluate analgesic activity of Terminalia bellerica fruit pulp aqueous extract by acetic acid induced writhing reflex test in Swiss albino mice by peripheral mechanism. Materials and Methods:Albino mice of Swiss strain (Male / Female-equal number), were `selected for the study and each mouse weighed about 15-30gms. Five experimental groups made assigned as Control, Standard and three groups of test drugs at different doses, and the doses were Gum acacia (1%), 10ml/kg, Diclofenac sodium (30mg/kg), Terminalia bellerica 9, 18 and 36 mg/kg respectively. All the drugs were administered orally one hour before commencing the experiment followed by injecting 0.1 ml of 0.6% acetic acid intraperitoneally and writhing reflex was observed for next five minutes. Observations recorded as mean ± SD. Results obtained were analyzed by using ANOVA (one-way) statistical test followed by Dunnet’s multiple comparison test and if p value Results: Our study reveals that aqueous extract of Terminalia bellerica fruit pulp in the dose of 9mg/kg (4.83±1.47=p Conclusion:Aqueous extract of Terminalia bellerica fruit pulp exhibited peripheral action in all the dose of 9, 18 and 36 mg/kg by acetic acid induced writhing reflex test in Swiss albino mice. Keywords: Aqueous extract, Fruit pulp, Peripheral analgesic activity, Terminalia bellerica, Writhing test