Analeptic efficacy of Tinospora cordifolia ethanolic leaf extract and its loaded Phytoniosome on enzymatic and non-enzymatic antioxidants in a rodent model of Polycystic ovary syndrome

Abstract

IntroductionPCOS (Polycystic ovary syndrome) is being known as a significant metabolic and reproductive disorder deliberating to increased risk for type 2 diabetes. Tinospora cordifolia is frequently used in traditional system of medicine where the leaf of the plant possesses pharmacological properties like hypoglycemic, anti-inflammatory and antioxidant activity. In recent years the Niosomes a novel drug delivery system is gaining more attention because of their biodegradability, toxicity reduction, improved therapeutic effectiveness and absorption enhancement. In our present study an attempt is made to examine the ameliorative effect of ethanolic leaf extracts of Tinospora cordifolia (ELETC) and Tinospora cordifolia loaded phytoniosomes (nELETC) on enzymatic and non-enzymatic antioxidants in PCOS female rats that are induced by mifepristone. Materials and MethodsPCOS was induced by administrating 4mg/kg b. w. Mifepristone to female rats for 8 days and confirmed by persistent estrous cycle. 200mg /kg b. w of ELETC and nELETC extracts were given to the rats which attained PCOS condition for 28 days. The enzymatic antioxidants SOD, CAT, GR, GST and GPx and non-enzymatic antioxidants GSH, ascorbic acid and α-Tocopherol were estimated followed by Histopathological analysis. ResultThe antioxidants showed decreased levels in the PCOS induced rats. The treatment with the drug nELETC reverted the antioxidant levels back to normalcy. From the histopathological studies we can understand that corpus luteum, antral follicles and primordial follicular cells were absent with the formation of follicular cyst in the mifepristone induced PCOS rats. nELETC drug treated rats showed the presence of corpus luteum with graafian follicles and primordial follicle cells, with the absence of follicular cyst, indicating nELETC could regain the functional ability of the ovaries from PCOS condition. ConclusionFrom the restoration of antioxidant status we can conclude that nELETC extract can be effectively used in the management of PCOS.