Anaerobic starvation realizes partial nitrification and starts anammox bacteria self-enrichment in mainstream municipal sewage treatment in a low filling ratio sequencing batch reactor

Abstract

The initiating and stable preservation of partial nitrification (PN) and achievement of anammox bacteria self-enrichment in domestic sewage is a purposeful subject. In this article, an originality tactics of anaerobic starvation for 100 days was adopted for rapidly achieving PN in actual wastewater, the nitrite accumulation rate (NAR) improved from 4.95% to 81.73% in 18 days. After anaerobic starvation was stopped, the stable PN effect furnished enough stroma for the growth of anammox bacteria. The abundance of Candidatus Brocadia grew from 0% to 0.42% in floc sludge and 0.43% in blank biofilm, which promoted nitrogen removal effect. Anaerobic starvation continuing 74 days generated further decrease in the abundance of Nitrobacter and Nitrospira of nitrite-oxidizing bacteria (NOB), indicating that anaerobic starvation can restore the destroyed partial nitrification. In conclusion, this article furnished a low-cost method for achieving anammox bacteria self-enrichment in mainstream municipal wastewater in 10% filling ratio without chemicals addition.