Anaerobic nitrate and ammonium metabolism in flood-tolerant rice coleoptiles

Abstract

The tolerance of germinating rice seedlings to anaerobiosis cannot be fully accounted for by ethanolic fer mentation alone. Nitrate metabolism (nitrate reduction to NH plus its subsequent assimilation) may provide an additional sink mechanism for excess protons and NADH produced during anaerobiosis. To follow the fate of nitrate, 15N-labelled nitrate and ammonium incorporation in aerobic and anaerobic rice coleoptiles was examined using 15N-edited 1H NMR and gas chromatography-mass spectrometry methods. After 22h of treatments, protein-free Ala, Glu, Gln, and γ-aminobutyrate were the main 15N-labelled products for both nitrate and ammonium-treated anaerobic rice coleoptiles, with Gln, Glu, and Ala being the most enriched. The total amount of 15N label incorporation into Ala and GAB increased significantly in response to anaerobiosis. The 15N-labelling pattern of Glu and Gln suggests that the GS/GOGAT system was primarily involved in ammonium assimilation whereas Glu dehydrogenase may play a role in nitrate assimilation. 15N incorporation into protein-derived amino acids was also significant and was more substantial in anaerobic than in aerobic rice coleoptiles, which indicate that protein biosynthesis remained active in anaerobic rice coleoptiles. Thus, anaerobic assimilation of inorganic N into amino acids, particularly Ala and Glu/GAB, may serve to supplement ethanolic fermentation in sustaining glycolysis and energy production in rice coleoptiles.