Abstract
Two types of raw-starch-digesting microorganisms were isolated from water and soil and were tentatively identified as Bacillus species. These strains were able to digest potato, corn and waxy starch in the rat state. They produced ethanol, butanol, acetate, butyrate, hydrogen and carbon dioxide from raw starch, the main products being ethanol, acetate and hydrogen gas. They were distinguished by the quantities of ethanol and hydrogen gas produced. Amylopectin was digested more vigorously than amylose. GPC spectra showed that a fermentation product with a molecular weight of several thousand daltons was formed in the culture broth, though intermediates of low molecular weight were not detected.
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