Abstract
An agar medium, designated X-Glu agar, was developed for the selective enumeration of Lactobacillus acidophilus in yogurt-related milk products containing a mixed microflora of lactobacilli, streptococci and bifidobacteria. The detection principle is based on the specific visualization of the β-d-glucosidase activity of L. acidophilus via a chromogenic reaction of 5-bromo-4-chloro-3-indolyl-β-d-glucopyranoside (X-Glu) which is incorporated into a Rogosa agar medium at 40 μg/ml. The X-Glu agar was more selective than other commonly used media (MRS. TGV, conventional Rogosa agar) in tests involving different bacterial test strains, yogurt and yogurt-related products.
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