An upregulated long noncoding RNA RP3‐337D23.3 in lung adenocarcinoma in never‐smokers promotes metastasis (1049.1)

Abstract

Objective: The present study was designed to screen and validate lung adenocarcinoma in never smokers (LACINS) specific long noncoding RNAs (lncRNAs).Methods: A custom‐designed human lncRNA expression microarray was utilized to determine lncRNA expression profiles of LACINS and indentify LACINS specific lncRNA. Five LACINS and paired adjacent normal lung tissues were used for microarray analysis and another 10 LACINS were used for further searching of LACINS specific lncRNAs by quantitative real‐time polymerase chain reaction. Function of RP3‐337D23.3 was investigated by cell proliferation, transwell, and wound healing assay in vitro.Results: A total of 3353 lncRNAs were differentially expressed in LACINS (fold change >2), 1509 upregulated and 1849 downregulated. Eleven candidate LACINS specific lncRNAs were identified and further validated in 10 pairs of LACINS and corresponding lung tissues. We identified RP3‐337D23.3 as a LACINS specific lncRNAs which is significantly up‐regulated in tumors as compared with adjacent normal tissues. Additionally, RP3‐337D23.3 is significantly overexpressed in LACINS with metastasis compared with those without metastasis (p=0.0006). In vitro siRNA mediated silencing of RP3‐337D23.3 led to remarkable decreased invasion, migration, and proliferation abilities of H1975 cell line.Conclusion: We identified the first LACINS specific lncRNA, RP3‐337D23.3, and provided preliminary evidence that RP3‐337D23.3 could promote LACINS metastasis.Grant Funding Source: National Natural Science Foundation of China (81372321)