Abstract
As well as the development of assisted reproductive technology (ART), as the current treatment of woman who failed in achieving pregnancy, the development of an advance vitrification method also grows rapidly. The successful of oocyte vitrification depends on the type and the concentration of cryoprotectant. This study was addressed to elaborate empirical evidence and recent studies of sucrose and trehalose as an extracellular CPA with the aim of achieving the success of oocyte vitrification. Several researchers in agreement that trehalose, as extracellular cryoprotectant, also has a role as intracellular cryoprotectant by microinjection with high survival rates as the outcome. Moreover, the combination of sucrose or trehalose as an extracellular cryoprotectant and others intracellular cryoprotectant have different survival rates which might occur because of the differences between the composition and concentration of sucrose or trehalose. The appropriate type and concentration of sugar as an extracellular cryoprotectant for oocyte cryopreservation are sucrose or trehalose in 0.5M concentration. Nevertheless, it requires further study to optimize oocyte vitrification process.
Highlights
The renewed highlight of current research is concerning on human oocyte cryopreservation
Following up to previous research, Eroglu et al.,9 assessed the freezing method using intracellular trehalose 0.15 M by microinjection combine with extracellular Dimethyl sulfoxide (DMSO) 0.5 M, outcoming 84% of mice oocyte survival rate
The undertaken study regarding the utilization of sugar as extracellular cryoprotectant performed good outcome, in the cryosurvival rate
Summary
The renewed highlight of current research is concerning on human oocyte cryopreservation. (Table 1) Recent data perform single trehalose or sucrose and the combination of both with another CPA, are being utilized either for a freezing method or vitrification. Following up to previous research, Eroglu et al., assessed the freezing method using intracellular trehalose 0.15 M by microinjection combine with extracellular DMSO 0.5 M, outcoming 84% of mice oocyte survival rate. Combination of sucrose and trehalose in the slow freezing method, by Borini et al., achieved 74.1% of oocyte survival rate. Elaborated study in oocyte vitrification, either the combination of cryoprotectant or concentration was varied such as propylene glycol, propanodiol and ethylene glycol, resulting the survival rate range from 65% to 92.43%. The previously researches were developed by using either freezing methods or vitrification with the utilization of single trehalose or combine to another CPA.
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