Abstract
Current methods for detection of mycotoxin in feed are time-consuming and tedious. An up-converting phosphor technology-based lateral flow (UPT-LF) assay system is a new emerging technique for analytes detection. The aim of this study was to compare the performance of UPT-LF, an enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for detecting aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON) in feed. The results showed that the use of UPT-LF for AFB1, ZEN and DON detection exhibited the following: limits of detection of 3, 50 and 200 μg/kg; average recoveries of 104.39%, 102.94% and 103.65%; and precision of 13.96%, 13.71% and 12.56%; respectively. UPT-LF required 45 min to determine one mycotoxin and 1.5 h to determine three mycotoxins in a sample, which took the shortest time. Besides, there were positive correlations between the UPT-LF, ELISA and HPLC/MS/MS methods. In conclusion, UPT-LF can be used to detect and quantify AFB1, ZEN and DON in feed samples. Though the sensitivity, accuracy and precision of UPT-LF are inferior to those of HPLC-MS/MS and ELISA, the UPT-LF assay is the most convenient and rapid technique for on-site detection among the three methods.
Highlights
Mycotoxins are secondary metabolites produced by filamentous fungi, especially those belonging to the genera Aspergillus, Penicillium, Alternaria, and Fusarium
The limit of detection (LOD) of aflatoxin B1 (AFB1), ZEN and DON by the upconverting phosphor technology-based lateral flow (UPT-LF) method were 3 μg/kg, 50 μg/kg, and 200 μg/kg, respectively, which were satisfactory for the detection requirements of the three mycotoxins in feed
Zhao et al [23] reported that the LOD of AFB1 in corn by UPT-LF was 3 μg/kg, which was similar to our results
Summary
Mycotoxins are secondary metabolites produced by filamentous fungi, especially those belonging to the genera Aspergillus, Penicillium, Alternaria, and Fusarium. Mycotoxins pose a high risk to the health of livestock and humans due to their toxigenic, carcinogenic, immunogenic, neurogenic, and mutagenic properties [1]. An up-converting phosphor technology-based lateral flow assay for mycotoxin detection in feed. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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