Abstract
A new polyketide containing the benzoisoquinoline-9-one moiety, peyronetide A (1), and three other new derivatives peyronetides B–D (2–4), as well as one known compound (5) were purified from the cultured broth of the endophytic fungus Peyronellaea sp. FT431, which was isolated from the Hawaiian indigenous plant, Verbena sp. The structures of the new compounds were determined through the analysis of HRMS and NMR spectroscopic data. Compounds 1, 2, and 5 showed cytotoxic activities against TK-10 (human kidney adenocarcinoma cells), cisplatin sensitive A2780S (human ovarian carcinoma cells), and cisplatin resistant A2780CisR cell lines, with IC50 values between 6.7 to 29.2 μM.
Highlights
Endophytic fungi are wonderful producers of various secondary metabolites, which have attracted great interest in the past decades to identify structurally unique and biologically active small molecules [1,2,3,4,5,6]
The fungal strain was isolated on PDA medium from a healthy leaf of a Hawaiian indigenous plant, Verbena sp., which was collected in the Lyon Botanical Garden in 2014
The strain FT431 was identified as Peyronellaea sp. based on the analysis of the DNA sequence of the nuclear ribosomal internal transcribed spacer, which has been deposited in GenBank with the accession no
Summary
Endophytic fungi are wonderful producers of various secondary metabolites, which have attracted great interest in the past decades to identify structurally unique and biologically active small molecules [1,2,3,4,5,6]. The crude extract of FT431 showed antiproliferative activity at 20 μg/mL against the A2780 cancer cell line, but verbenanone was inactive, so we decided to study FT431 further to identify the antiproliferative compounds. The fermented whole broth (4.5 L) was filtered through filter paper to separate the supernatant from the mycelia. The latter was extracted with 80% acetone/H2 O (×3), and the extract was concentrated under reduced pressure to afford an aqueous solution. Molecules 2019, 24, 196 against different cancer cell lines. We report the isolation, structure elucidation, and bioactivities of these isolated compounds
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