Abstract
The effects of Actinomycin D (Act D) and cycloheximide (CHI) on ethylene (ETH)-mediated abscission were studied, using detached panicles of tobacco flowers. CHI reversed ETH-mediated abscission; Act D did not. Both inhibitors caused most mitochondria to develop large electron-transparent areas and some cristae disorganization. Act D caused the development of numerous membrane whorls that averaged 2 μm in diameter and caused areas of the plasma membrane to pull away from the cell wall, revealing possible sites of attachment of the wall to the plasma membrane. Many microbodies in Act D-treated tissue also became polymorphic and developed electron-dense matrices. Cells of control, CHI-, CHI-and-ETH-, and Act D-treated tissue contained small amounts of rough endoplasmic reticulum (RER). The ETH- and Act D-and-ETH-treated abscission cells had substantially elevated levels of RER; with both treatments, abscission was accelerated.
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