Abstract
In this paper, a sensitive immunosensor was designed using copper tungstate@molybdenum sulfide (CuWO4@MoS2) and chitosan-gold (Chit-Au) nanocomposites to detect cortisol. CuWO4@MoS2 nanocomposites was synthesized by hydrothermal synthesis, gold nanoparticles (AuNPs) were synthesized by a sodium citrate reduction, and Chit-Au was synthesized by ultrasonic method. The manifestation platform of x-ray diffraction (XRD), transmission electron microscopy (TEM), and energy disperse spectroscopy (EDS), and scanning electron microscopy (SEM) were devoted to characterizing morphologies and structures of the obtained nanocomposites. Then, Chit-Au and CuWO4@MoS2 were modified in GCE to obtain the Chit-Au/CuWO4@MoS2/GCE electrode. Cortisol antibody (C-Mab) was successfully combined with Chit-Au/CuWO4@MoS2/GCE electrode using 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N–H-hydroxy succinimide (NHS). Furthermore, the immunosensor was characterized using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques, and the cortisol levels were evaluated by square wave voltammetry (SWV) technique in a PBS (pH 7.0, 0.9% NaCl) solution comprising 5 mM [Fe(CN)6]3−/4−. The results demonstrate that the immunosensor exhibited a wide ranges from 0.1 fg/mL to 1 μg/mL and a low detection limit of 0.014 fg/mL in cortisol detection.
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