Abstract
The ferricyanide test, which was originally developed for the determination of blood sugar, was applied to 17 other sugars in addition to glucose. Although sensitivity differed, all free reducing sugars gave a positive test. Substances without a free reducing group (inositol, glucose-1-phosphate, and sucrose) did not react. Acetaldehyde also failed to giv a positive test. Deoxy sugars and phosphorylated sugars gave less color than free sugars with a full complement of hydroxy groups. Standard curves, prepared for 10 sugars, indicated that Beer's law is followed reasonably well. The presence of cellulose fibers in suspension did not interfere with the ferricyanide test. It was also possible to hydrolyze sucrose in 0.5 N sulfuric acid without significant hydrolysis of cellulose fibers washed from filter paper. Adherence to Beer's law, freedom from interference by cellulose, and extreme sensitivity (0.01 μmole of sugar) are advantages which should m ake the modified ferricyanide test well suited for quantitative chromatography of many sugars.
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