Abstract
This study has developed a sensitive and simple ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of corydaline, dehydrocorydaline, tetrahydropalmatine, protopine, palmatine, tetrahydroberberine, columbamine, berberine, coptisine and berberrubine in beagle dog plasma after the oral administration of the Corydalis yanhusuo W.T. Wang and Yuanhuzhitong tablets. Chromatographic separation was achieved on an Agilent Eclipse Plus C18 RRHD column (1.8 µm, 50 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection was conducted by multiple reaction monitoring (MRM) mode via an electrospray ionization source in the positive mode. The calibration curves of all analytes showed good linear (r2 > 0.9800). The intra-day and inter-day precisions were less than 15% and the accuracies were within ±15%. The extraction recoveries conformed to the acceptable range. And there was no interference of endogenous substances in the sensitive assay method. All analytes were proven to be stable during sample storage and analysis procedures. The pharmacokinetic study indicated that the Yuanhuzhitong tablets could get a better absorption than Corydalis yanhusuo W.T. Wang.
Highlights
Yuanhuzhitong tablet (YHZT), formally incorporated into Chinese Pharmacopoeia and ChineseNational Essential Medicine List, consists of two herbs including 223 g of Radix Angelicae dahuricae and 445 g of vinegar-processed Corydalis yanhusuo W.T
Alkaloids and coumarins are the main active constituents of YHZT, which possess a variety of pharmacological actions, such as analgesia, spasmolysis, anti-inflammatory, antianxiety and vasodilatation [2]
electrospray ionization (ESI) operated in positive mode was preferentially selected as ESIOwing operated in positive modesamples was preferentially selected as themass ionization source forConsequently, the compounds
Summary
National Essential Medicine List, consists of two herbs including 223 g of Radix Angelicae dahuricae and 445 g of vinegar-processed Corydalis yanhusuo W.T. Wang (C. yanhusuo) [1]. Alkaloids and coumarins are the main active constituents of YHZT, which possess a variety of pharmacological actions, such as analgesia, spasmolysis, anti-inflammatory, antianxiety and vasodilatation [2]. The vinegar-quenching processing technology has been utilized for the extraction of the active ingredient of C. yanhusuo due to a better solubility and to increase the curative effect [3]. The processing method of vinegar C. yanhusuo has a long application history in traditional Chinese medicines (TCM). Previous study showed that the alkaloid content of C. yanhusuo was higher with vinegar in its decoction process than that without it [4]. Clinical practice and previous pharmacological studies have proven that Radix
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