An SNP Mutation of Gene RsPP Converts Petal Color From Purple to White in Radish (Raphanus sativus L.).

Dongming Liu,Luming Yang,Xiaochun Wei,Lin Li,Jinfang Liang,Yuxiang Yuan,Zhiyong Wang,Yanyan Zhao,Henan Su,Xiaowei Zhang,Shuangjuan Yang,Dongling Sun

doi:10.3389/fpls.2021.643579

Abstract

Along with being important pigments that determining the flower color in many plants, anthocyanins also perform crucial functions that attract pollinators and reduce abiotic stresses. Purple and white are two different colors of radish petals. In this study, two cDNA libraries constructed with purple and white petal plants were sequenced for transcriptome profiling. Transcriptome results implied that the expression level of the genes participating in the anthocyanin biosynthetic pathway was commonly higher in the purple petals than that in the white petals. In particular, two genes, F3′H and DFR, had a significantly higher expression pattern in the purple petals, suggesting the important roles these genes playing in radish petal coloration. BSA-seq aided-Next Generation Sequencing of two DNA pools revealed that the radish purple petal gene (RsPP) was located on chromosome 7. With additional genotyping of 617 F2 population plants, the RsPP was further confined within a region of 93.23 kb. Transcriptome and Sanger sequencing analysis further helped identify the target gene, Rs392880. Rs392880 is a homologous gene to F3′H, a key gene in the anthocyanin biosynthetic pathway. These results will aid in elucidating the molecular mechanism of plant petal coloration and developing strategies to modify flower color through genetic transformation.

Highlights

Anthocyanins are a group of glycosylated polyphenolic compounds widely present in plant tissues; they confer color to them, varying from orange, red, and purple, to blue
The total anthocyanin content of the ZYR1 petals was significantly higher than that of HYR3 petals, some anthocyanin accumulation was observed in HYR3 (Figure 1C)
In ZYR1 and HYR3, we found a dramatic reduction of anthocyanin content, implying a close relationship between the petal color appearance and pigment content changes (Figure 1C)

Summary

Introduction

Anthocyanins are a group of glycosylated polyphenolic compounds widely present in plant tissues; they confer color to them, varying from orange, red, and purple, to blue. To protect the plants from environmental stress, anthocyanins may help fight pathogens or act as UV screens and antioxidants via accumulation in specialized cells (Treutter, 2005). One of their most essential functions is influencing petal coloration, which is integral to the successful attraction of pollinators and seed distributors Petal coloration varies with changes in PH and structural modifications of the anthocyanidins (Tanaka et al, 1998)

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Conclusion