Abstract
AbstractA reverse transcription‐polymerase chain reaction (RT‐PCR)‐based assay for the detection of tomato spotted wilt virus (TSWV) has been modified and improved by the use of immunocapture of virus by solid phase adsorbed antibodies and colorimetric detection of nested PCR products, into which digoxigenin has been incorporated, by an ELOSA (enzyme‐linked oligonucleotidesorbent assay)‐like assay. The use of the colorimetric assay for the detection of tospoviruses by using ‘universal’ primers in the RT‐PCR stage is also described.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
