Abstract
In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from −141 to −162 of the rat α1(I) procollagen promoter with a Kdof 0.1-0.2 μM. The formation of triplexes by the triplex forming oligoribonucleotide (ORN) was also observed under physiological conditions.In vitrotranscription run-off experiments showed that triplex formation results in inhibited transcription from the rat α1(I) procollagen gene. Our results demonstrate a novel approach for down-regulation of procollagen gene transcriptionin vivo.
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