Abstract
The design and construction of an oxygenation-perfusion apparatus, used in a study on neonatal enteritis in calves, as well as the experimental procedure employed, are illustrated and described. The method provides a means of quantitating net directional transport or absorption of perfusion solvent and solutes across the intestinal walls of large mammals. Further, it provides for a controlled rate of flow of perfusion fluid through the intestinal segment. Essentially, the method consists of independent mucosal and serosal systems, both bathed by a modified Krebs-Ringer solution and both continuously oxgenated. A peristaltic pump controls the circulation rate of the mucosal solution. The serosal solution may be either constantly mixed by gas pressure or circulated by another pump. Provisions for convenient sampling and for rapid apparatus assembly and disassembly are provided. The method was successfully used with 33 intestinal segments without apparent tissue damage after 75 minutes of experimentation.
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