Abstract
An Oxalate oxidase (Oxalate: O2 oxidoreductase, EC 1,2,3,4) has been purified to apparent homogeneity from leaves of 10-day old seedling plants of grain sorghum hybrid CSH-5. The enzyme exhibited maximum activity at pH 5.0 and 40°C. The rate of H2O2 formation was linear upto 2 min. The enzyme was strongly stimulated by Cu++. The enzyme has greater resistance towards various cations and anions found in urine, compared to moss, barley, banana peel and bleet stem oxalate oxidases. This improved characteristic of the enzyme make it better suited for its use in the determination of urinary oxalate. A simple method of measuring oxalate in urine using this enzyme preparation is described.
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