Abstract
Yeast surface display has become an increasingly popular tool for protein engineering and library screening applications. Although, recent advances have greatly expanded the capability of yeast surface display, the protein display system is still far away from industrial application. One of the major components of a stable, efficient and successful yeast surface display system is cell wall anchor protein with which our desired foreign protein will be attached. We studied 80 different yeast cell wall anchored proteins originated mostly from Saccharomyces cerevisiae and Candida albicans. We studied in details all the cell wall proteins in order to find out suitable cell wall proteins to recommend for the researchers to use in the construction of yeast display system. We considered selective physical properties of different yeast cell wall proteins that are crucial for selecting best suited cell surface anchor proteins which are molecular weight, binding domain of anchor protein, length of amino acid and fusion site. Finally, our studies showed that Ccw11, Ccw12. Cwp1, Cwp2, Dan1, Gas1, Gas5, Exg1, Ycr89, Ecm33, Pga4, Sap9, Sap10, Pst1, Pir1, Pir2, Pir3, Pir4, Cis1, Scw4, Scw6, Bgl2, Uth1, Scw1 are the promising and suitable cell wall anchor proteins could be used in construction of yeast cell surface display system. Additionally, this review presents detailed information about all the cell wall proteins in a single work. The future researchers in this field will be able to construct more efficient yeast display system for recombinant protein production at industrial scale using the knowledge presented in this work.
 Asian J. Med. Biol. Res. June 2019, 5(4): 246-257
Highlights
Surface display of proteins by incorporation into the cell walls of different microorganisms is a method established in the last two decades as a promising way of creation of new tools for modern biotechnology
We found many yeast cell wall proteins that were unused for constructing yeast surface display system
The desired foreign protein, we want to display on the cell surface of yeast cell wall, must be attached to the anchor protein
Summary
Surface display of proteins by incorporation into the cell walls of different microorganisms is a method established in the last two decades as a promising way of creation of new tools for modern biotechnology. GPI-anchored proteins comprise the largest group of yeast cell wall protein These are covalently attached cell wall proteins linked to β-1, 3-glucan through the remnants of glycosylphosphatidylinositol (GPI) anchors and β-1, 6-glucan (Klis et al, 2006.). Other group of covalently linked cell wall proteins are Pir proteins which are linked to β-1,3-glucan through alkali labile ester linkages Based on these binding approaches of cell wall proteins, the surface display systems in the yeast could be classified into three categories: Flo system, GPI system and Pir system. GPI-anchored proteins destined for the cell wall are liberated from the plasma membrane by cleavage of their anchors This lipidless GPI remnants of GPI–CWPs become linked to the external surface of the β-1, 3-glucan network indirectly through β-1, 6-glucan chains.
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