An Overview of the Medical Applications of Marine Skeletal Matrix Proteins.

M Rahman

doi:10.3390/md14090167

Abstract

In recent years, the medicinal potential of marine organisms has attracted increasing attention. This is due to their immense diversity and adaptation to unique ecological niches that has led to vast physiological and biochemical diversification. Among these organisms, marine calcifiers are an abundant source of novel proteins and chemical entities that can be used for drug discovery. Studies of the skeletal organic matrix proteins of marine calcifiers have focused on biomedical applications such as the identification of growth inducing proteins that can be used for bone regeneration, for example, 2/4 bone morphogenic proteins (BMP). Although a few reports on the functions of proteins derived from marine calcifiers can be found in the literature, marine calcifiers themselves remain an untapped source of proteins for the development of innovative pharmaceuticals. Following an overview of the current knowledge of skeletal organic matrix proteins from marine calcifiers, this review will focus on various aspects of marine skeletal protein research including sources, biosynthesis, structures, and possible strategies for chemical or physical modification. Special attention will be given to potential medical applications and recent discoveries of skeletal proteins and polysaccharides with biologically appealing characteristics. In addition, I will introduce an effective protocol for sample preparation and protein purification that includes isolation technology for biopolymers (of both soluble and insoluble organic matrices) from coralline algae. These algae are a widespread but poorly studied group of shallow marine calcifiers that have great potential for marine drug discovery.

Highlights

Skeletal proteins and polysaccharides in marine organisms are present as complex mixtures within organic matrices
Macromolecules derived from marine calcifiers that hold promise for biomedical applications include a broad range of protein and sugar molecules that participate in signaling, development, regeneration, and metabolism
If purification methods can be established for skeletal proteins derived from calcifying marine organisms, researchers in the emerging fields of proteomics and medicinal chemistry could utilize these methods for subsequent drug discovery and, as a more specific example, bone repair

Summary

Introduction

Skeletal proteins and polysaccharides in marine organisms are present as complex mixtures within organic matrices. Proteins with potential for bone repair and drug discovery, extracted either from naturally occurring skeletal organic matrices or derived from cultivated tissues, can be identified and isolated using chromatography, cell assays and proteomic methods [1,9,11]. If purification methods can be established for skeletal proteins derived from calcifying marine organisms, researchers in the emerging fields of proteomics and medicinal chemistry could utilize these methods for subsequent drug discovery and, as a more specific example, bone repair. The information with respect to the close homology of soft coral and human proteins provides us functional and evolutionary clues on the structure and functions of their sequences These homologous proteins could lead to possible drug discovery and form a potential resource for biotechnological research. Some significant chemical reactions of acylation, alkylation, Schiff base formation and reductive N-alkylation, carboxyalkylation, N-phthaloylation are well described [35]

A Promising Future for Marine Calcifiers in Drug Discovery

2, References

Electrophoretic

Findings

Conclusions