Abstract

In juice processing, ultrasound treatment has been tested as a potential alternative to conventional thermal methods to inactivate microorganisms and to enhance the nutritional status of juice. In this study, the impact of pasteurization and high-power ultrasound treatment on the quality of red grape juice was investigated in terms of the content of bioactive compounds such as phenolic compounds and l-ascorbic acid as well as regarding the microbiological and physicochemical properties. The grape juice was subjected to pasteurization (80 °C, 2 min) as well as to ultrasound treatment with an amplitude of 50 and 70% for 5 and 10 min. The results indicated the same level of total phenolic content for pasteurized and sonicated samples for 10 min with an amplitude of 70%, while the highest level of l-ascorbic acid was recorded for sonicated samples with an amplitude of 70% for 10 min. pH of sonicated samples decreased with amplitude and treatment time while total soluble solids and titratable acidity increased with amplitude and time. Moreover, the results indicated the usefulness of juice sonication to enhance the inactivation of microorganisms. Thus, the high-power ultrasound treatment might represent a viable technique to replace the conventional thermal treatment in grape juice processing.

Highlights

  • Grapes are one of the largest fruit crops harvested around the world

  • The effects of pasteurization and high-power ultrasound (HPU) treatments on the quality of red grape juice are discussed in terms of bioactive compounds such as total phenolic (TP) content, l-ascorbic acid (L-AsAc) or vitamin C content and the profile of polyphenolic compounds

  • All mentioned parameters were investigated for untreated red grape juice, as a control sample (C), as well as for red grape juice samples subjected to pasteurization at 80 ◦ C for 2 min (P), respectively to sonication with an amplitudes of 50 and 70% for 5 and 10 min, as follows: HPU (A50%, 5 min), HPU (A50%, 10 min), HPU (A70%, 5 min) and HPU (A70%, 5 min)

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Summary

Introduction

Grapes are one of the largest fruit crops harvested around the world. grape juice is not consumed worldwide in large amounts because of its higher sugar content or higher acidity, but it can be mixed with apple or other juices [1,2,3]. The last decades were characterized by numerous studies related to the chemical constituents of grapes, especially phenolic compounds that are secondary plant metabolites with free radical scavenging activity [4,5]. Anthocyanins, flavanols, flavonols and dihydroflavonols are considered to play an important role in the pigmentation and Molecules 2020, 25, 1669; doi:10.3390/molecules25071669 www.mdpi.com/journal/molecules. Molecules 2020, 25, 1669 flavor of grapes berries, while non-flavonoid phenolic compounds such as resveratrol have a role in the powerful biological activities of grape [6,7]. It was considered that a diet based on a high daily intake of antioxidant dietary supplements from fruits and vegetables might result in a lower incidence of atherosclerosis, Alzheimer’s disease, cancer, ocular disease, diabetes, rheumatoid arthritis and motor neuron disease [8]

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