Abstract
The histones are shown to be unequally extracted from nuclei or isolated chromatin of Chinese hamster ovary cells. To realise an overall study of phosphorylation of histone variants, we have developed an analytical system in which the five main histones are first separated on a sodium dodecyl sulfate gel then, after electroelution analysed on a Triton acid urea gel. Thus, no specific loss of histones is detected. In CHO cells, the following histone variants are phosphorylated : 5 % of the non acetylated and the monoacetylated H4 - around 30 % of H2A1 and H2A2 - less than 2 % of H2B1 - H31 and its monoacetylated form are the main phosphorylated variants of the three H3. All the H1 molecules are phosphorylated both in H11 and H12.
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