Abstract
To assess the viability of cultured epithelium and preserved by freezing for periods varying from one month to one year. Samples of cultured epithelium were incubated in cryoprotectant medium (Group A), packed in aluminum envelopes and packed in polystyrene boxes. The boxes were subjected to a temperature of-70 ºC. After freezing for a period of time ranging from one to 12 months, cultured epithelial samples were assessed for their viability by vital staining (Trypan blue) and metabolic analysis based on glucose consumption and lactate production. Samples of not frozen cultured epithelium (Group B) were also tested for viability and the results obtained were used as comparison parameter for the variation of viability. Statistical analysis between the group A and B indicate that the mean age of the donors (p=0.51) and the culture time (p=1.18) showed no statistical difference. In 30 days we obtained 37% of the original viability of cultured epithelium, 25% at six months and one year, less than 15%. This trend was confirmed statistically with a reduction of approximately 1.8% of the original viability epithelium cultured every 30 days of storage. In the analysis by lactate production, similar results were observed. In the analysis by the glucose consumption results were not significant. The viability indices show statistically significant difference between the group A and B (p<0.0001). Although cryopreserved cultured epithelium showed significant reduction of viability, all samples remained viable. It was also found that the viability of cryopreserved cultured epithelial decreased as a function of storage time.
Highlights
An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts
Introduced by Rheinwald and Green[1], the use of cultured epidermal autografts in burns has been based on a technique for effective keratinocyte cultures[1]
Contrary, cultured epidermal allografts are applicable anytime when necessary, and they were expected to be an important method in burn treatment
Summary
An outcome analysis and long-term viability of cryopreserved cultured epidermal allografts. The possibility to transfer skin has greatly improved the restoration of tissue defects following traumas, clinical disease or major traumas[1,2,3,4,5,6,7,8,9]. Human skin allografts can be an alternative and the wound bed can be temporary covered. Allografts are indicated to prepare the wound before the use of definitive cultured epidermal autografts, in order to provide a permanent dermal replacement underneath the epidermal sheets[10,11]. Cultured epidermal are taken as a permanent coverage of wounds, and they are known as an epoch-making treatment for large burns for which an adequate skin replacement is complex to achieve[4,13]. The process is more efficient when the epithelium is produced and stored in a tissue bank to be used promptly
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
