Abstract
A unique assay method to detect ornithine decarboxylase (ODC) activity in normal tracheal epithelial cells induced by a classic tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) has been developed. Using this new assay, the inhibition of induced ODC activity is measured, thereby providing a screening system to identify potential chemopreventive or therapeutic agents. The assay is designed to be specific as it detects a radioactively labeled reaction product, putrescine from the decarboxylation of ornithine by ODC. As TPA is a classical tumor promoter, inhibition TPA-induced ODC activity can also be used to select antitumor promoter agents. Since the assay requires only 2 days to complete, it can be used to screen a large number of compounds for chemopreventive activity in a relatively short period of time.
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