Abstract
Adult and foetal guinea pig skin from a number of sites including metatarsal pad, tongue, ear and abdomen can be maintained as full thickness explants showing negligible necrosis for up to seven days in vitro. The skin pieces are explanted on to acetate rayon gauze supported by stainless steel mesh rafts contained in widemouthed 500 ml flasks with a fluid medium. High concentrations of O 2 and 5 per cent CO 2 give the best skin survival; the most satisfactory medium tested consists of calf serum, embryo extract 1 and an amino acid vitamin supplement with a high glucose concentration. Histological comparisons have been made of the skin in vitro and in vivo and the similarities and differences discussed. Epidermal differentiation, migration and phagocytosis take place very much as in vivo but changes similar to an injury response occur, namely, cell hypertrophy and reduced basophilia together with a cessation of mitotic activity. No mitotic stimulant has yet been found.
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