Abstract
Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, however, the material basis responsible for the therapeutic benefits remains largely unclear. This study aimed to provide an optimized method for extracting and characterizing phenolic compounds in D. indicum var. aromaticum flower. Firstly, an ultrasound-assisted method combined with central composite circumscribed (CCC) design was applied to optimize phenolic compound extraction. Ethanol-acetic acid (70%:2%, v/v) was selected as solvent, and the optimal extraction condition was: extraction temperature, 57 °C; solid/liquid ratio, 1:30 g/mL; extraction time, 20 min. Secondly, an effective and economic HPLC-PDA-ESI-MSn method was established and validated for phenolic compound characterization and quantification. As a result, 14 phenolic compounds were identified, including 8 phenolic acids and 6 flavonoids, and for the first time, oleuropein derivatives, chrysoeriol, and tricin are reported in D. indicum var. aromaticum flower. The content of phenolics identified by HPLC-MSn was 6.42 ± 0.32 mg/g DW. The optimized method for extraction and characterization of phenolic compounds has significant meaning to future pharmaceutical and medicinal research on D. indicum var. aromaticum, and the results in this study can provide references for herbal research.
Highlights
Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, the material basis responsible for the therapeutic benefits remains largely unclear
To better explore values of D. indicum var. aromaticum in scientific research and in medicinal resource development, it makes sense to figure out the phenolic profile in D. indicum var. aromaticum
Ultrasound-assisted extraction method was optimized by CCC design, and under optimal condition
Summary
Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, the material basis responsible for the therapeutic benefits remains largely unclear. Aromaticum plant has been widely used as herbal medicine in China, the material basis responsible for the therapeutic benefits remains largely unclear. This study aimed to provide an optimized method for extracting and characterizing phenolic compounds in D. indicum var. The optimized method for extraction and characterization of phenolic compounds has significant meaning to future pharmaceutical and medicinal research on D. indicum var. Many in vivo and in vitro investigations have revealed that intake of some phenolic compounds especially certain flavonoids contributes to the prevention of hypertension. Many in vivo and in vitro investigations have revealed that intake of some phenolic compounds especially certain flavonoids contributes to the prevention of hypertension6,7 All these studies provide theoretical basis for the therapeutic benefits of D. indicum var. To better explore values of D. indicum var. aromaticum in scientific research and in medicinal resource development, it makes sense to figure out the phenolic profile in D. indicum var. aromaticum
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