Abstract
The quality of the X-ray tomogram not only depends on the spatial resolution but also on the density resolution or contrast. Based on the theory of [Nucl. Instr. and Meth. 206 (1983) 541] it is concluded that the density resolution can be substantially improved by merging of pixels, referred to as binning, prior to reconstruction. We demonstrate that the quality of a given 3D-data set, i.e. the tomogram of stained biological cells seeded on a polymer multifilament yarn in phosphate buffered saline, can be optimized with respect to the product of spatial and density resolution – the image quality factor. This procedure improves or even enables the visualization and quantification of selected constituents in the tomogram.
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Schedule a callMore From: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
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